coli cells and was not cytotoxic for macrophages but had an inhibitory effect on the growth of B. Scramble peptide without CRAC motifs did not inhibit the growth of E. The half-maximal inhibitory concentration (IC50) for B. subtilis strains at concentrations that are significantly lower than the cytotoxic concentration that was found for macrophages. In the present work, we have found that peptide P4 inhibits the growth of E. Previously we have shown that 50 μM of peptide P4 is toxic to cultured mouse macrophages. In this work, we studied the antibacterial activity of a peptide named P4 with the following sequence RTKLWEMLVELGNMDKAVKLWRKLKR that was constructed from two alpha-helical fragments of the influenza virus protein M1 and containing two cholesterol-recognizing amino-acid consensus (CRAC) motifs. Among them are the effects that AMPs cause on bacterial cell membranes. Diverse mechanisms underlie the antibacterial action of AMPs. Due to the emergence of multiple antibiotic resistance in many pathogens, the studies on new antimicrobial peptides (AMPs) have become a priority scientific direction in fundamental and applied biology.
0 Comments
Leave a Reply. |